Development of an amperometric biosensor based on acetylcholine esterase covalently bound to a new support material

Summary, in English

A new type of amperometric biosensor based on immobilised acetylcholine esterase was designed and constructed. The enzyme was immobilised on a flow-through working electrode, which was prepared from reticulated vitreous carbon (RVC) or from a composite material consisting of RVC and superporous agarose. The sensor was operated in FIA mode using acetylthiocholine as a substrate. The sensor responded to inhibitors such as paraoxon—10−9 mol was detected by the sensor in a non-optimised configuration. The practical lifetime of the sensor was at least 1 month.

• Författare: M Khayyami; M T Pérez Pita; N Peña Garcia; Gillis Johansson; Bengt Danielsson; Per-Olof Larsson
• Avdelning/ar: Centrum för analys och syntes; Tillämpad biokemi
• Publiceringsår: 1998
• Språk: Engelska
• Sidor: 557-563
• Publikation/Tidskrift/Serie: Talanta
• Volym: 45
• Issue: 3
• Länkar: http://dx.doi.org/10.1016/S0039-9140(97)00182-3
• Dokumenttyp: Artikel i tidskrift
• Förlag: Elsevier
• Ämne: Composite Science and Engineering; Analytical Chemistry
• Nyckelord: Acetylthiocholine; Acetylcholine esterase; Biosensor; RVC-electrode; Amperometric; Thiocholine; Mediator; Superporous agarose gel
• Aktiv: Published
• ISBN/ISSN/Övrigt: ISSN: 1873-3573